Journal: Frontiers in Immunology

Article Title: Zika virus induces monocyte recruitment in the immunocompetent adult brain driving chronic inflammation

doi: 10.3389/fimmu.2025.1597776

Figure Lengend Snippet: ZIKV results in activated microglia with a change in morphology. (A) CX3CR1-GFP mice were injected with ZIKV at 10^6 PFU via footpad, brains were harvested at 0 and 7dpi. (B) 100X immunofluorescence imaging of mouse cortex staining for DAPI and CX3CR1. (C) 100X immunofluorescence of mouse cortex to assess microglial morphology using sall1 and CX3CR1-GFP. (D) PCR data for CD86, CX3CR1 and CH25H genes, N=3. Data represents the mean ± SD ∗ p < 0.0332, ∗∗ p < 0.0021. The experiment was independently repeated twice with similar results; representative data are shown.

Article Snippet: Primary antibodies used were SALL1 (eBioscience), and GFAP (sc33673), and secondary antibodies used were AF647 (Invitrogen).

Techniques: Injection, Immunofluorescence, Imaging, Staining

Journal: Frontiers in Immunology

Article Title: Zika virus induces monocyte recruitment in the immunocompetent adult brain driving chronic inflammation

doi: 10.3389/fimmu.2025.1597776

Figure Lengend Snippet: Infiltrating monocytes seen in ZIKV mice brains at 7dpi. (A) CCR2-CreER;R26R-EGFP (Ai6) mice were given tamoxifen for 3 days before infected with ZIKV and harvested. (B) Quantification of flow data for Bone marrow cells (CCR2-EGFP+, CCR2-EGFP+ CD68+) (C) Quantification of flow data for Brain cells (CD11B+CD45+, CD45int CD11B+ (Microglia), CCR2-EGFP+, CCR2-EGFP-). (D, E) Immunofluorescence of CCR2-CreER-R26R-EGFP mice for EGFP and sall1 expression in choroid plexus region with quantification. N=3-4. Data represents the mean ± SD ∗ p < 0.0332, ∗∗ p < 0.0021, and ∗∗∗ p < 0.0002.

Article Snippet: Primary antibodies used were SALL1 (eBioscience), and GFAP (sc33673), and secondary antibodies used were AF647 (Invitrogen).

Techniques: Infection, Immunofluorescence, Expressing

Journal: bioRxiv

Article Title: Senescent-like microglia limit remyelination through the senescence associated secretory phenotype

doi: 10.1101/2024.05.23.595605

Figure Lengend Snippet: a, Quantification of colocalization of p16tdt and cell markers via ImageJ percent area (n= 6-8). b-c, Immunofluorescent staining and Imaris 3D reconstruction of p16tdt and SALL1 . d, Heatmap depicting Damage associate microglia (DAM) gene expression in young lesions relative to young non-lesions (n=3). Color represents LogFC relative to young non-lesion and * indicates significantly differentially expressed. Data are mean ± SEM ( a ). P values derived from one-way ANOVA with Tukey corrected multiple comparisons ( a ) or Wald test ( d ).

Article Snippet: Slides were then blocked for 1 hour at RT in blocking/antibody buffer (5% Donkey serum, 1%BSA, 0.4% Triton X) before being stained overnight at 4C with the following primary antibodies: mouse anti-APC (CC1) (Abcam #ab16794, 1:100), goat anti-CCL11 (R&DSystems #AF-420-SP, 1:50), rat anti-Cd11b (Biorad #MCA74G, 1:100), CD68 (Biolegend #137020, 1:100), rat anti-Clec7a (InvivoGen #mabg-mdect, 1:100), mouse anti-GFAP (Millipore Sigma #G3893, 1:500), rabbit anti-Iba1 (Fujifilm Wako #019-19741, 1:400), mouse anti-iNos (BD Biosciences #610329, 1:100), rat anti-MBP (Biorad #MCA409S, 1:500), mouse anti-Nkx2.2 (DSHB #74.5A5-c, 1:100), rabbit anti-Olig2 (Millipore Sigma #AB9610, 1:300), mouse anti-p16 (Abcam #ab54210, 1:500), mouse anti-p21 (Santa Cruz #sc6246, 1:500), rabbit anti-RFP (Rockland Immunochemicals #600-401-379, 1:1000), rat anti-Sall1 (Invitrogen #14-9729-82).

Techniques: Staining, Expressing, Derivative Assay