Journal: Biomedical Journal

Article Title: Correlation of SALL1 with CEUS parameters and immune escape in thyroid carcinoma

doi: 10.1016/j.bj.2025.100829

Figure Lengend Snippet: Analysis of DEGs in the GSE65144 dataset using GEO2R. (A) Identification of 152 DEGs in thyroid carcinoma, visualized with a volcano plot (showing upregulated and downregulated genes) and a heat map highlighting expression differences. (B) PPI networks for upregulated and downregulated DEGs, illustrating key gene interactions within TC. (C) Functional enrichment analysis of selected DEGs using GO and KEGG pathways, with emphasis on genes associated with pituitary gland function, including SALL1, NKX2-1, and CDH1, to identify pathways relevant to TC progression and immune response.

Article Snippet: For transfection, lentiviral vectors targeting SALL1 were designed by Shanghai Genechem Co. Ltd. and used to create the following experimental groups: the NC group (non-transfected control), the OV group (overexpression vector to increase SALL1 expression), and the SI group (silencing vector to knock down SALL1 expression).

Techniques: Expressing, Functional Assay

Journal: Biomedical Journal

Article Title: Correlation of SALL1 with CEUS parameters and immune escape in thyroid carcinoma

doi: 10.1016/j.bj.2025.100829

Figure Lengend Snippet: Analysis of SALL1 expression and diagnostic potential in TC. (A) GEPIA analysis showing low SALL1 expression in CESC, KICH, OV, and high in GBM, LGG. (B) SALL1 expression in TC with no impact on prognosis. (C) Higher SALL1 positivity in TC tissues than adjacent tissues ( p < 0.05). (D) Elevated SALL1 protein in TC tissues ( p < 0.05). (E) Increased SALL1 mRNA in TC ( p < 0.05). (F) ROC curve showing SALL1 mRNA >1.69 as a diagnostic marker for TC with 65.52% sensitivity and 93.10% specificity ( p < 0.05).

Article Snippet: For transfection, lentiviral vectors targeting SALL1 were designed by Shanghai Genechem Co. Ltd. and used to create the following experimental groups: the NC group (non-transfected control), the OV group (overexpression vector to increase SALL1 expression), and the SI group (silencing vector to knock down SALL1 expression).

Techniques: Expressing, Diagnostic Assay, Marker

Journal: Biomedical Journal

Article Title: Correlation of SALL1 with CEUS parameters and immune escape in thyroid carcinoma

doi: 10.1016/j.bj.2025.100829

Figure Lengend Snippet: Analysis of the relationship between SALL1 expression and immune cell infiltration in TC. (A) Correlation between SALL1 expression and various immune cell types, showing significant associations with B cells, CD8 + T cells, and macrophages, but minimal correlation with CD4 + T cells, neutrophils, and dendritic cells. (B) Kaplan-Meier survival analysis indicates that SALL1 expression does not significantly impact TC patient survival in the context of immune cell infiltration. (C) Changes in SALL1 copy number can lead to altered levels of immune cell types within the tumor microenvironment.

Article Snippet: For transfection, lentiviral vectors targeting SALL1 were designed by Shanghai Genechem Co. Ltd. and used to create the following experimental groups: the NC group (non-transfected control), the OV group (overexpression vector to increase SALL1 expression), and the SI group (silencing vector to knock down SALL1 expression).

Techniques: Expressing

Journal: Biomedical Journal

Article Title: Correlation of SALL1 with CEUS parameters and immune escape in thyroid carcinoma

doi: 10.1016/j.bj.2025.100829

Figure Lengend Snippet: Correlation between SALL1 and CEUS imaging parameters in TC. (A) CEUS parameter comparison between TC and non-TC patients, with significantly lower PI, TTP, and MTT in TC. ∗ indicates p < 0.05. (B) CEUS parameters across TC stages, showing a progressive decrease with advanced stages (∗, #, & indicate p < 0.05 for comparisons with stages I, II, and III, respectively). (C) CEUS parameter differences in TC patients with and without lymph node metastasis ( p < 0.05). (D) Positive correlation of SALL1 mRNA levels with PI, TTP, and MTT ( p < 0.05).

Article Snippet: For transfection, lentiviral vectors targeting SALL1 were designed by Shanghai Genechem Co. Ltd. and used to create the following experimental groups: the NC group (non-transfected control), the OV group (overexpression vector to increase SALL1 expression), and the SI group (silencing vector to knock down SALL1 expression).

Techniques: Imaging, Comparison

Journal: Biomedical Journal

Article Title: Correlation of SALL1 with CEUS parameters and immune escape in thyroid carcinoma

doi: 10.1016/j.bj.2025.100829

Figure Lengend Snippet: Impact of SALL1 on TC progression . (A) GFP staining confirming SALL1 transfection. (B) Verification of SALL1 expression and levels of autophagy and pyroptosis markers. (C) Cloning assay showing SALL1's effect on TC cell proliferation. (D) Transwell assay assessing SALL1's impact on cell invasion. (E) Flow cytometry analysis of SALL1-induced apoptosis. (F) Flow cytometry of cell cycle alterations due to SALL1. (G) SALL1 expression in vivo confirming intervention efficacy. N = 3 in all cases (H) In vivo tumor growth assessment. ∗, # indicate p < 0.05 compared to NC and OV groups, respectively.

Article Snippet: For transfection, lentiviral vectors targeting SALL1 were designed by Shanghai Genechem Co. Ltd. and used to create the following experimental groups: the NC group (non-transfected control), the OV group (overexpression vector to increase SALL1 expression), and the SI group (silencing vector to knock down SALL1 expression).

Techniques: Staining, Transfection, Expressing, Cloning, Transwell Assay, Flow Cytometry, In Vivo

Journal: Biomedical Journal

Article Title: Correlation of SALL1 with CEUS parameters and immune escape in thyroid carcinoma

doi: 10.1016/j.bj.2025.100829

Figure Lengend Snippet: Influence of SALL1 on the immune microenvironment in TC cells. (A) Fluorescence intensity of PD-1 showed reduced expression in the SALL1 OV group and increased expression in the SI group. (B) Fluorescence intensity of PD-L1 with similar patterns, reduced in the OV group and elevated in the SI group. (C) Proportional analysis of immune cell types showed increased CD4 + T and B cells and decreased MDSC levels in the OV group, with opposite trends in the SI group. n = 3. ∗, # indicate p < 0.05 compared with NC and OV groups, respectively.

Article Snippet: For transfection, lentiviral vectors targeting SALL1 were designed by Shanghai Genechem Co. Ltd. and used to create the following experimental groups: the NC group (non-transfected control), the OV group (overexpression vector to increase SALL1 expression), and the SI group (silencing vector to knock down SALL1 expression).

Techniques: Fluorescence, Expressing

Journal: Biomedical Journal

Article Title: Correlation of SALL1 with CEUS parameters and immune escape in thyroid carcinoma

doi: 10.1016/j.bj.2025.100829

Figure Lengend Snippet: SALL1 associated with pituitary function regulates immune escape of TC cells.

Article Snippet: For transfection, lentiviral vectors targeting SALL1 were designed by Shanghai Genechem Co. Ltd. and used to create the following experimental groups: the NC group (non-transfected control), the OV group (overexpression vector to increase SALL1 expression), and the SI group (silencing vector to knock down SALL1 expression).

Techniques:

Journal: Frontiers in Immunology

Article Title: Zika virus induces monocyte recruitment in the immunocompetent adult brain driving chronic inflammation

doi: 10.3389/fimmu.2025.1597776

Figure Lengend Snippet: ZIKV results in activated microglia with a change in morphology. (A) CX3CR1-GFP mice were injected with ZIKV at 10^6 PFU via footpad, brains were harvested at 0 and 7dpi. (B) 100X immunofluorescence imaging of mouse cortex staining for DAPI and CX3CR1. (C) 100X immunofluorescence of mouse cortex to assess microglial morphology using sall1 and CX3CR1-GFP. (D) PCR data for CD86, CX3CR1 and CH25H genes, N=3. Data represents the mean ± SD ∗ p < 0.0332, ∗∗ p < 0.0021. The experiment was independently repeated twice with similar results; representative data are shown.

Article Snippet: Primary antibodies used were SALL1 (eBioscience), and GFAP (sc33673), and secondary antibodies used were AF647 (Invitrogen).

Techniques: Injection, Immunofluorescence, Imaging, Staining

Journal: Frontiers in Immunology

Article Title: Zika virus induces monocyte recruitment in the immunocompetent adult brain driving chronic inflammation

doi: 10.3389/fimmu.2025.1597776

Figure Lengend Snippet: Infiltrating monocytes seen in ZIKV mice brains at 7dpi. (A) CCR2-CreER;R26R-EGFP (Ai6) mice were given tamoxifen for 3 days before infected with ZIKV and harvested. (B) Quantification of flow data for Bone marrow cells (CCR2-EGFP+, CCR2-EGFP+ CD68+) (C) Quantification of flow data for Brain cells (CD11B+CD45+, CD45int CD11B+ (Microglia), CCR2-EGFP+, CCR2-EGFP-). (D, E) Immunofluorescence of CCR2-CreER-R26R-EGFP mice for EGFP and sall1 expression in choroid plexus region with quantification. N=3-4. Data represents the mean ± SD ∗ p < 0.0332, ∗∗ p < 0.0021, and ∗∗∗ p < 0.0002.

Article Snippet: Primary antibodies used were SALL1 (eBioscience), and GFAP (sc33673), and secondary antibodies used were AF647 (Invitrogen).

Techniques: Infection, Immunofluorescence, Expressing